• Featured Product
  • KD/KO Validated

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20854-1-AP

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

20854-1-AP targets NSUN2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight 767 aa, 86 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC001041
Gene symbol NSUN2
Gene ID (NCBI) 54888
RRIDAB_10693629
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Protocols

Product Specific Protocols
WB protocol for NSUN2 antibody 20854-1-APDownload protocol
IHC protocol for NSUN2 antibody 20854-1-APDownload protocol
IF protocol for NSUN2 antibody 20854-1-APDownload protocol
IP protocol for NSUN2 antibody 20854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis

Authors - Sunyang Ying
humanWB

Nat Biotechnol

Identification of direct targets and modified bases of RNA cytosine methyltransferases.

Authors - Khoddami Vahid V
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
humanWB,IHC

Nat Cell Biol

5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.

Authors - Xin Chen
humanWB

Nat Protoc

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Authors - Vahid Khoddami
humanWB,IF

Cell Host Microbe

Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression.

Authors - David G Courtney
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (01-05-2021)

Works perfectly for WB. Tested on HeLa cells and HEK293T.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa and HEL293T cells