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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NRBP2 Monoclonal antibody

NRBP2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F10H7

Cat no : CL488-66687

Synonyms

DKFZp434P086, NRBP2, pp9320, TRG 16, TRG16

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using CL488-66687

    IF Staining of HEK-293 using CL488-66687

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488-conjugated NRBP2 antibody (CL488-66687, Clone: 4F10H7 ) at dilution of 1:100.

  • IF Staining of HeLa using CL488-66687

    IF Staining of HeLa using CL488-66687

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66687 (NRBP2 antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells, HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66687-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66687 targets NRBP2 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NRBP2 fusion protein Ag17011 相同性解析による交差性が予測される生物種
Full Name nuclear receptor binding protein 2
Calculated molecular weight 501 aa, 58 kDa
Observed molecular weight 58 kDa, 33 kDa
GenBank accession numberBC113873
Gene symbol NRBP2
Gene ID (NCBI) 340371
RRIDAB_2883367
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear receptor binding protein 2(NRBP2), also named as TRG16, belongs to the Ser/Thr protein kinase family. NRBP2 can regulate apoptosis of neural progenitor cells during their differentiation. Expression of NRBP2 was up-regulated following differentiation in rodent neural stem/progenitor cells. It has 4 isoforms with MW of 58, 33, 36 and 14 kDa. Endogenous NRBP2 in HEK293 cells has an apparent molecular mass of 55-60 kD by SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NRBP2 antibody CL488-66687Download protocol
Standard Protocols
Click here to view our Standard Protocols