Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells, L02 cells, mouse liver tissue, rat liver tissue, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
Product Information
12235-1-AP targets NR4A1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2875 Product name: Recombinant human NR4A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-598 aa of BC016147 Sequence: KYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKQPPDASPANLLTSLVRAHLDSGPSTAKLDYSKFQELVLPHFGKEDAGDVQQFYDLLSGSLEVIRKWAEKIPGFAELSPADQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHRLQCARGFGDWIDSILAFSRSLHSLLVDVPAFACLSALVLITDRHGLQEPRRVEELQNRIASCLKEHVAAVAGEPQPASCLSRLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPPIIDKIFMDTLPF 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear receptor subfamily 4, group A, member 1 |
| Calculated molecular weight | 598 aa, 64 kDa |
| Observed molecular weight | 64-70 kDa |
| GenBank accession number | BC016147 |
| Gene Symbol | NR4A1 |
| Gene ID (NCBI) | 3164 |
| RRID | AB_10644125 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22736 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NR4A1 is an orphan nuclear receptor that act concomitantly with NURR1 in regulating the expression of delay-early genes during liver regeneration. Also NR4A1 has linked not only to cell apoptosis but also proliferation in response to growth factor. It plays a role in caspase-independent cell death by activation of the ERK pathway and increased activity of MEF2 transcription factors. NR4A1 exists some isoforms with MW 65 and 34 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| IP protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| WB protocol for NR4A1 antibody 12235-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Metab HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma
| ||
Exp Mol Med NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis | ||
J Adv Res Nur77 attenuates Paneth cell necroptosis-induced intestinal inflammation through regulating endoplasmic reticulum homeostasis in sepsis | ||
J Adv Res SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice. | ||
J Nanobiotechnology Enhancing antitumor immunity in triple-negative breast cancer through reversal of T-cell exhaustion using a CD8-targeted and membrane-camouflaged nanocarrier delivering small interfering RNA against sorting Nexin 9. | ||
Adv Sci (Weinh) A Graphene Composite Film Based Wearable Far-Infrared Therapy Apparatus (GRAFT) for Effective Prevention of Postoperative Peritoneal Adhesion |