Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse kidney using 21854-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21854-1-AP
WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells.
WB analysis using 21854-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 21854-1-AP
IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug.
IHC staining of human kidney using 21854-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21854-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 21854-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 21854-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 21854-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 21854-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 21854-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IF Staining of HEK-293 using 21854-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21854-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Synonyms

MCR, MLR, MR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HEK-293 cells, HeLa cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue, human colon tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 23 publications below
IHC	See 11 publications below
IF	See 8 publications below
ChIP	See 3 publications below

Product Information

21854-1-AP targets NR3C2 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16410 Product name: Recombinant human NR3C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC111758 Sequence: METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQLVKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAIVKSPIMCHEKSPSVCSPLNMTSSVCSPAGINSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCSVKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSA 相同性解析による交差性が予測される生物種
Full Name	nuclear receptor subfamily 3, group C, member 2
Calculated molecular weight	984 aa, 107 kDa
Observed molecular weight	94-110 kDa
GenBank accession number	BC111758
Gene Symbol	NR3C2
Gene ID (NCBI)	4306
RRID	AB_10858785
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08235
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Protocols

Product Specific Protocols
IF protocol for NR3C2 antibody 21854-1-AP	Download protocol
IHC protocol for NR3C2 antibody 21854-1-AP	Download protocol
IP protocol for NR3C2 antibody 21854-1-AP	Download protocol
WB protocol for NR3C2 antibody 21854-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IHC	J Pineal Res Melatonin prevents experimental central serous chorioretinopathy in rats. Authors - Shanshan Yu View Article
human	WB	EMBO Rep High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction. Authors - Yukang Yuan View Article
rat	WB	Oncotarget Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats. Authors - Shuai Lian View Article
rat	IHC,IF	Int Immunopharmacol UUO induces lung fibrosis with macrophage-myofibroblast transition in rats. Authors - Fan Yang View Article
rat	WB	Behav Brain Res Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats. Authors - Shuai Lian View Article
rat	WB	Behav Brain Res Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats. Authors - Shuai Lian View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 21854-1-AP

WB analysis of HEK-293 using 21854-1-AP

WB analysis using 21854-1-AP

IP experiment of HEK-293 using 21854-1-AP

IHC staining of human kidney using 21854-1-AP

IHC staining of human kidney using 21854-1-AP

IHC staining of human colon using 21854-1-AP

IHC staining of human colon using 21854-1-AP

IHC staining of mouse kidney using 21854-1-AP

IHC staining of mouse kidney using 21854-1-AP

IF Staining of HepG2 using 21854-1-AP

IF Staining of HEK-293 using 21854-1-AP

NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Melatonin prevents experimental central serous chorioretinopathy in rats.

High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.