• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NR2F6 Monoclonal antibody

NR2F6 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H2B8

Cat no : 60117-2-Ig

Synonyms

EAR 2, EAR2, ERBAL2, NR2F6, V erbA related protein 2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 60117-2-Ig

    WB analysis of HeLa using 60117-2-Ig

    WB result of NR2F6 antibody (60117-2-Ig, 1:1000) with si-Control and si-NR2F6 transfected HeLa cells.

  • WB analysis using 60117-2-Ig

    WB analysis using 60117-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 60117-2-Ig

    WB analysis of HeLa using 60117-2-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 60117-2-Ig

    IHC staining of human ovary tumor using 60117-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 60117-2-Ig

    IHC staining of human kidney using 60117-2-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 60117-2-Ig

    IHC staining of human kidney using 60117-2-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human ovary tumor using 60117-2-Ig

    IHC staining of human ovary tumor using 60117-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HT-29 cells, LNCaP cells, MCF-7 cells, HEK-293 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman ovary tumor tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60117-2-Ig targets NR2F6 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR2F6 fusion protein Ag7614 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 2, group F, member 6
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC002669
Gene symbol NR2F6
Gene ID (NCBI) 2063
RRIDAB_2155653
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development [PMID:15875024]. NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) [12690040]. It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity [PMID:18701084].

Protocols

Product Specific Protocols
WB protocol for NR2F6 antibody 60117-2-IgDownload protocol
IHC protocol for NR2F6 antibody 60117-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Sci Adv

Melanoma-intrinsic NR2F6 activity regulates antitumor immunity

Authors - Hyungsoo Kim
View Article
humanWB

Nat Commun

Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.

Authors - Victoria Klepsch
  • KD Validated
View Article
humanWB

Biology (Basel)

Low-Dose Ionizing Radiation-Crosslinking Immunoprecipitation (LDIR-CLIP) Identified Irradiation-Sensitive RNAs for RNA-Binding Protein HuR-Mediated Decay

Authors - Ji Won Lee
View Article