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  • KD/KO Validated

NPC1 Polyclonal antibody

NPC1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13926-1-AP

Synonyms

Niemann Pick C1 protein, Niemann Pick disease, type C1, NPC1



Tested Applications

Positive WB detected inunboiled HEK-293 cells, unboiled mouse brain tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

13926-1-AP targets NPC1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPC1 fusion protein Ag4946 相同性解析による交差性が予測される生物種
Full Name Niemann-Pick disease, type C1
Calculated molecular weight 142 kDa
Observed molecular weight 160-200 kDa
GenBank accession numberBC063302
Gene symbol NPC1
Gene ID (NCBI) 4864
RRIDAB_2152050
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the functions of these two proteins within lysosomes are linked closely. NPC1 is a typical transmembrane protein and contains a number of modification sites for glycosylation. Defects in NPC1 are the cause of Niemann-Pick disease type C1 which exhibits highly variable clinical phenotype. Moreover, NPC1 may play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.

Protocols

Product Specific Protocols
WB protocol for NPC1 antibody 13926-1-APDownload protocol
IHC protocol for NPC1 antibody 13926-1-APDownload protocol
IF protocol for NPC1 antibody 13926-1-APDownload protocol
FC protocol for NPC1 antibody 13926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.

Authors - Bei-Bei Chu
  • KD Validated
humanWB

Nat Commun

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Authors - Yunkai Zhu
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
humanWB

Proc Natl Acad Sci U S A

Cholesterol trafficking is required for mTOR activation in endothelial cells.

Authors - Xu Jing J
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.

Authors - Constance S Petit
mouseWB

Cell Rep

The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.

Authors - Jian Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ganesh (Verified Customer) (02-08-2024)

25 µg of HAP1 (human) whole cell lysate was resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. NPC1 antibody was diluted at 1:1000 in Blocking Buffer (5% Milk in 1x TBS-T (0.1% Tween-20)) and the membrane was incubated overnight at 4 deg C on a rotator.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HAP1
NPC1 Antibody Western Blot validation (1:1000 dilution) in HAP1 (Cat no:13926-1-AP)
FH

Benjamin (Verified Customer) (12-09-2021)

Nice antibody that detects Npc1 at the correct molecular weight in primary mouse neurons.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Primary Mouse Neurons
NPC1 Antibody Western Blot validation (1:1000 dilution) in Primary Mouse Neurons (Cat no:13926-1-AP)