NOX5 Polyclonal antibody

NOX5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, PLA, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25350-1-AP

Synonyms

NADPH oxidase 5, NOX5, NOX5A, NOX5B



Tested Applications

Positive WB detected inJurkat cells, Raji cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25350-1-AP is NOX5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOX5 fusion protein Ag18673 相同性解析による交差性が予測される生物種
Full Name NADPH oxidase, EF-hand calcium binding domain 5
Calculated molecular weight 765 aa, 86 kDa
Observed molecular weight82-86 kDa
GenBank accession numberBC125098
Gene symbol NOX5
Gene ID (NCBI) 79400
RRIDAB_2811208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOX5 (NADPH oxidase, EF-hand calcium binding domain 5) is an NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca(2+)-dependent manner. It plays an important role in PDGF-induced JAK/STAT activation and human aortic smooth muscle cell (HASMC) proliferation. NOX5 is the main source of superoxide and is implicated in human spermatozoa motility. This protein has 6 isoforms produced by alternative splicing, named NOX5-α, -β, -γ, -δ, -ε and -ζ with the molecular mass of 84, 82, 86, 85, 64 and 83 kDa (PMID: 22427510 and Uniprot).

Protocols

Product Specific Protocols
WB protocol for NOX5 antibody 25350-1-APDownload protocol
IHC protocol for NOX5 antibody 25350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

Chronic hypoxia of endothelial cells boosts HIF-1α-NLRP1 circuit in Alzheimer's disease

Authors - Eunyoung Jung
monkey,humanWB

Free Radic Biol Med

Nitric oxide and heme-NO stimulate superoxide production by NADPH oxidase 5.

Authors - Elizabeth A Sweeny
humanWB,CoIP

Front Cell Dev Biol

Functional interactions between NADPH oxidase 5 and actin

Authors - Samantha M Richter
humanWB

Ecotoxicol Environ Saf

Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity.

Authors - Liling Su
humanWB,CoIP,PLA

Biomed Pharmacother

Therapeutic effect of phycocyanin on acute liver oxidative damage caused by X-ray.

Authors - Qi Liu
humanWB

Biochem Biophys Res Commun

miR-485 suppresses inflammation and proliferation of mesangial cells in an in vitro model of diabetic nephropathy by targeting NOX5.

Authors - Jian Wu