Validation Data Gallery
Tested Applications
| Positive WB detected in | bEnd.3 cells, HUVEC cells, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 99 publications below |
| IHC | See 16 publications below |
| IF | See 17 publications below |
Product Information
27120-1-AP targets eNOS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25712 Product name: Recombinant human NOS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAP 相同性解析による交差性が予測される生物種 |
| Full Name | nitric oxide synthase 3 (endothelial cell) |
| Observed molecular weight | 133 kDa, 69 kDa |
| Gene Symbol | eNOS |
| Gene ID (NCBI) | 4846 |
| RRID | AB_2880764 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29474 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for eNOS antibody 27120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Circulation Aberrant Phase Separation of Endothelial MAML1 Causes Congenital Heart Disease by Suppressing Notch Activity | ||
Nat Commun Metabolomics and proteomics reveal blocking argininosuccinate synthetase 1 alleviates colitis in mice | ||
J Adv Res OTUD1 delays wound healing by regulating endothelial function and angiogenesis in diabetic mice | ||
J Adv Res Plasma-activated solutions prevent peritoneal adhesion formation by regulating eNOS expression in mesothelial cells | ||
Redox Biol Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy | ||
Redox Biol Non-steroidal mineralocorticoid receptor antagonist finerenone ameliorates mitochondrial dysfunction via PI3K/Akt/eNOS signaling pathway in diabetic tubulopathy |