• Featured Product
  • KD/KO Validated

NONO Polyclonal antibody

NONO Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11058-1-AP

Synonyms

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB



Tested Applications

Positive WB detected inPC-3 cells, human pancreas tissue, human skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, HEK-293 cells, PC-12 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11058-1-AP targets NONO in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NONO fusion protein Ag1512 相同性解析による交差性が予測される生物種
Full Name non-POU domain containing, octamer-binding
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC010049
Gene symbol NONO
Gene ID (NCBI) 4841
RRIDAB_2152167
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Protocols

Product Specific Protocols
WB protocol for NONO antibody 11058-1-APDownload protocol
IHC protocol for NONO antibody 11058-1-APDownload protocol
IF protocol for NONO antibody 11058-1-APDownload protocol
IP protocol for NONO antibody 11058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
humanIF

Mol Cell

Condensates induced by transcription inhibition localize active chromatin to nucleoli.

Authors - Takaaki Yasuhara
humanWB,RIP

Adv Sci (Weinh)

The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA

Authors - Ya-Rui Hou
  • KD Validated
humanIF

Hepatology

Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.

Authors - Zhixiang Hu
humanWB,IHC,IF

Sci Adv

Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer.

Authors - Yuanyuan Qin
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was decent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: 293T
NONO Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11058-1-AP)
FH

Tatyana (Verified Customer) (05-14-2023)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
NONO Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11058-1-AP)