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  • Featured Product
  • KD/KO Validated

NOL6 Polyclonal antibody

NOL6 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16361-1-AP

Synonyms

NOL6, NRAP, Nucleolar protein 6, UTP22

Validation Data Gallery

Applications:
  • WB analysis of A431 using 16361-1-AP

    WB analysis of A431 using 16361-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16361-1-AP (NOL6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 16361-1-AP

    WB analysis of A431 using 16361-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16361-1-AP (NOL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 16361-1-AP

    IF Staining of A431 using 16361-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 16361-1-AP (NOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16361-1-AP targets NOL6 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL6 fusion protein Ag8368 相同性解析による交差性が予測される生物種
Full Name nucleolar protein family 6 (RNA-associated)
Calculated molecular weight 1146 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC008852
Gene symbol NOL6
Gene ID (NCBI) 65083
RRIDAB_2151993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOL6 antibody 16361-1-APDownload protocol
IF protocol for NOL6 antibody 16361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Epigenomics

NOL6 promotes the proliferation and migration of endometrial cancer cells by regulating TWIST1 expression.

Authors - Junhui Liang
  • KO Validated
View Article