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- KD/KO Validated
NOB1 Polyclonal antibody
NOB1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10091-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, SGC-7901 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lymphoma tissue, human placenta tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
10091-2-AP targets NOB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NOB1 fusion protein Ag0141 相同性解析による交差性が予測される生物種 |
| Full Name | NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47-50 kDa |
| GenBank accession number | BC000050 |
| Gene symbol | NOB1 |
| Gene ID (NCBI) | 28987 |
| RRID | AB_2298640 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOB1 antibody 10091-2-AP | Download protocol |
| IHC protocol for NOB1 antibody 10091-2-AP | Download protocol |
| IF protocol for NOB1 antibody 10091-2-AP | Download protocol |
| IP protocol for NOB1 antibody 10091-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2. | ||
Elife A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly. | ||
Elife Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation. | ||
World J Gastroenterol NOB1 is essential for the survival of RKO colorectal cancer cells.
| ||
Oncol Rep NOB1 silencing inhibits the growth and metastasis of laryngeal cancer cells through the regulation of JNK signaling pathway. | ||
Oncol Lett Effects of NOB1 on the pathogenesis of osteosarcoma and its expression on the chemosensitivity to cisplatin.
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