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NNMT Polyclonal antibody
NNMT Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15123-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | L02 cells, HepG2 cells, mouse liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
15123-1-AP targets NNMT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NNMT fusion protein Ag7197 相同性解析による交差性が予測される生物種 |
| Full Name | nicotinamide N-methyltransferase |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC000234 |
| Gene symbol | NNMT |
| Gene ID (NCBI) | 4837 |
| RRID | AB_2153579 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NNMT antibody 15123-1-AP | Download protocol |
| IF protocol for NNMT antibody 15123-1-AP | Download protocol |
| IP protocol for NNMT antibody 15123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids | ||
Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. | ||
Mol Oncol Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.
| ||
Front Cell Infect Microbiol Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells. | ||
Sci Rep The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease. | ||
Life Sci Glucose availability regulates nicotinamide N-methyltransferase expression in adipocytes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (01-05-2024) | This antibody worked for immunofluorescence with a little background
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FH Johnny (Verified Customer) (01-07-2022) | products from proteintech have always been reliable.
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