Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
28571-1-AP targets NMDAR2A/GRIN2A in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29423 Product name: Recombinant human NMDAR2A/GRIN2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1000-1127 aa of NM_000833 Sequence: VSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPG 相同性解析による交差性が予測される生物種 |
| Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 2A |
| Calculated molecular weight | 165 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | NM_000833 |
| Gene Symbol | GRIN2A |
| Gene ID (NCBI) | 2903 |
| RRID | AB_2881171 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12879 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NMDAR2A/GRIN2A antibody 28571-1-AP | Download protocol |
| IHC protocol for NMDAR2A/GRIN2A antibody 28571-1-AP | Download protocol |
| WB protocol for NMDAR2A/GRIN2A antibody 28571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Behav Brain Res Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice | ||
Heliyon Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1 | ||
Neurochem Res Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression | ||
J Ethnopharmacol Anticonvulsant effect of Stachydrine on pentylenetetrazole-induced kindling seizure mouse model via Notch and NMDAR signaling pathways | ||
Neuron The paraventricular thalamus mediates visceral pain and anxiety-like behaviors via two distinct pathways |
