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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse NK1.1 (CD161) (PK136)

NK1.1 (CD161) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

PK136

Cat no : CL647-65138

Synonyms

CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a

Validation Data Gallery

Applications:
  • FC experiment of mouse splenocytes using CL647-65138

    FC experiment of mouse splenocytes using CL647-65138

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with FITC Anti-Mouse CD49b (DX5) and 0.25 ug CoraLite® Plus 647-conjugated Anti-Mouse NK1.1 (CD161) (CL647-65138, Clone: PK136) or 0.25 ug CoraLite® Plus 647-conjugated Mouse IgG2a isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65138-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65138 targets NK1.1 (CD161) in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen NK-1+ cells from mouse spleen and bone marrow 相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily B member 1A
GenBank accession numberBC120707
Gene symbol Klrb1a
Gene ID (NCBI) 17057
RRIDAB_2883704
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 NK1.1 (CD161) antibody CL647-65138Download protocol
Standard Protocols
Click here to view our Standard Protocols