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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NICN1 Monoclonal antibody

NICN1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B7G6

Cat no : CL488-67328

Synonyms

NICN1, nicolin 1, PGs5

Validation Data Gallery

Applications:
  • FC experiment of HEK-293 using CL488-67328

    FC experiment of HEK-293 using CL488-67328

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NICN1 (CL488-67328, Clone:5B7G6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67328-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67328 targets NICN1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NICN1 fusion protein Ag6581 相同性解析による交差性が予測される生物種
Full Name nicolin 1
Calculated molecular weight 24 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC050653
Gene symbol NICN1
Gene ID (NCBI) 84276
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 NICN1 antibody CL488-67328Download protocol
Standard Protocols
Click here to view our Standard Protocols