Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, HeLa cells, human heart tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
15370-1-AP targets NFS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3872 Product name: Recombinant human NFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC018471 Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF 相同性解析による交差性が予測される生物種 |
| Full Name | NFS1 nitrogen fixation 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC018471 |
| Gene Symbol | NFS1 |
| Gene ID (NCBI) | 9054 |
| RRID | AB_2151933 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y697 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NFS1 antibody 15370-1-AP | Download protocol |
| IHC protocol for NFS1 antibody 15370-1-AP | Download protocol |
| IP protocol for NFS1 antibody 15370-1-AP | Download protocol |
| WB protocol for NFS1 antibody 15370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. | ||
J Nanobiotechnology Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation. | ||
PLoS One Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.
| ||
Antioxidants (Basel) Downregulation of Iron-Sulfur Cluster Biogenesis May Contribute to Hyperglycemia-Mediated Diabetic Peripheral Neuropathy in Murine Models |
