Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, HeLa cells, human heart tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue, human pancreas cancer tissue, human testis tissue, human ovary tissue, mouse liver tissue, rat liver tissue, human kidney tissue, human placenta tissue, rat brain tissue, human brain tissue, human stomach cancer tissue, human spleen tissue, mouse brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
15370-1-AP targets NFS1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3872 Product name: Recombinant human NFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC018471 Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF 相同性解析による交差性が予測される生物種 |
| Full Name | NFS1 nitrogen fixation 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC018471 |
| Gene Symbol | NFS1 |
| Gene ID (NCBI) | 9054 |
| RRID | AB_2151933 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y697 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NFS1 antibody 15370-1-AP | Download protocol |
| IHC protocol for NFS1 antibody 15370-1-AP | Download protocol |
| IP protocol for NFS1 antibody 15370-1-AP | Download protocol |
| WB protocol for NFS1 antibody 15370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. | ||
Redox Biol NFS1, together with FXN, protects cells from ferroptosis and DNA damage in diffuse large B-cell lymphoma. | ||
Redox Biol Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation | ||
J Nanobiotechnology Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation. | ||
Adv Sci (Weinh) Impaired Iron-Sulfur Cluster Synthesis Induces Mitochondrial PARthanatos in Diabetic Cardiomyopathy | ||
Adv Sci (Weinh) NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2.
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