Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of K-562 using 11089-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11089-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11089-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11089-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 11089-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11089-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 11089-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11089-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 11089-1-AP
IP result of anti-NFE2 (IP:11089-1-AP, 4ug; Detection:11089-1-AP 1:500) with K-562 cells lysate 3440ug.
IHC staining of human colon cancer using 11089-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11089-1-AP (NFE2 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 11089-1-AP
Immunofluorescent analysis of HepG2 cells, using NFE2 antibody 11089-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

NFE2 Polyclonal antibody

NFE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11089-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

p45 NF E2, p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, U-937 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IHC	See 1 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

11089-1-AP targets NFE2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1570 Product name: Recombinant human NFE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC005044 Sequence: MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD 相同性解析による交差性が予測される生物種
Full Name	nuclear factor (erythroid-derived 2), 45kDa
Calculated molecular weight	41 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC005044
Gene Symbol	NFE2
Gene ID (NCBI)	4778
RRID	AB_2152928
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16621
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.

Protocols

Product Specific Protocols
IF protocol for NFE2 antibody 11089-1-AP	Download protocol
IHC protocol for NFE2 antibody 11089-1-AP	Download protocol
IP protocol for NFE2 antibody 11089-1-AP	Download protocol
WB protocol for NFE2 antibody 11089-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Am Coll Cardiol Heme levels are increased in human failing hearts. Authors - Khechaduri Arineh A View Article
mouse,human		Cell Rep Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism Authors - Hao Qin View Article
human,mouse	WB,CoIP	Life Sci Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys. Authors - Shunying Jin KD Validated View Article
human	WB	Life Sci ERK and p38 MAPK inhibition controls NF-E2 degradation and profibrotic signaling in renal proximal tubule cells. Authors - Jia Li View Article
human	WB	BMC Genomics Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages. Authors - Carme Arnan View Article
mouse	WB	Adv Sci (Weinh) Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation. Authors - Zhiyuan Lu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of K-562 using 11089-1-AP

WB analysis of K-562 using 11089-1-AP

WB analysis of K-562 using 11089-1-AP

WB analysis of K-562 using 11089-1-AP

WB analysis of U-937 using 11089-1-AP

WB analysis of K-562 using 11089-1-AP

WB analysis of U-937 using 11089-1-AP

WB analysis of K-562 using 11089-1-AP

IP experiment of K-562 using 11089-1-AP

IHC staining of human colon cancer using 11089-1-AP

IF Staining of HepG2 using 11089-1-AP

NFE2 Polyclonal antibody

NFE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Heme levels are increased in human failing hearts.

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.

ERK and p38 MAPK inhibition controls NF-E2 degradation and profibrotic signaling in renal proximal tubule cells.

Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages.

Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.