CoraLite® Plus 488-conjugated NF45 Monoclonal antibody
NF45 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A3A2
Cat no : CL488-67685
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67685-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-67685 targets NF45 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NF45 fusion protein Ag30388 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin enhancer binding factor 2, 45kDa |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC000382 |
| Gene symbol | ILF2 |
| Gene ID (NCBI) | 3608 |
| RRID | AB_2919519 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NF45 antibody CL488-67685 | Download protocol |
| FC protocol for CL Plus 488 NF45 antibody CL488-67685 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
