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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NEIL3 Polyclonal antibody

NEIL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11621-1-AP

Synonyms

DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease 8 like 3, Endonuclease VIII like 3, FGP2, FPG2, hFPG2, hNEI3, Nei like protein 3, NEI3, NEIL3

Validation Data Gallery

Applications:
  • WB analysis using 11621-1-AP

    WB analysis using 11621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11621-1-AP

    WB analysis of HEK-293 using 11621-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11621-1-AP

    WB analysis of HEK-293 using 11621-1-AP

    WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells.

  • WB analysis of human skin using 11621-1-AP

    WB analysis of human skin using 11621-1-AP

    human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11621-1-AP

    WB analysis of HEK-293 using 11621-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skin using 11621-1-AP

    WB analysis of mouse skin using 11621-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 11621-1-AP

    WB analysis of A431 using 11621-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

11621-1-AP targets NEIL3 in WB, IP, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEIL3 fusion protein Ag2200 相同性解析による交差性が予測される生物種
Full Name nei endonuclease VIII-like 3 (E. coli)
Calculated molecular weight 605 aa, 68 kDa
Observed molecular weight 62-68 kDa
GenBank accession numberBC025954
Gene symbol NEIL3
Gene ID (NCBI) 55247
RRIDAB_2251229
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

Protocols

Product Specific Protocols
WB protocol for NEIL3 antibody 11621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.

Authors - Dan Liu
View Article
humanWB

Nucleic Acids Res

Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.

Authors - Niu Li
  • KD Validated
View Article
humanWB

EMBO J

PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.

Authors - Daniel González-Acosta
View Article
humanWB

Sci Rep

NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells

Authors - Umit Aliyaskarova
View Article
humanWB,IP

DNA Repair (Amst)

Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.

Authors - Neurauter Christine Gran CG
View Article
humanWB,FC,IP,ChIP

Cell Rep

NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.

Authors - Jia Zhou
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jia (Verified Customer) (09-19-2019)

Good antibody for detecting NEIL3 in human cells.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:100 for IP, 1:500 for WB