Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 21698-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 21698-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 21698-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21698-1-AP
WB result of NEDD4 antibody (21698-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEDD4 transfected HeLa cells.
WB analysis of HT-1080 using 21698-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 21698-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of C6 using 21698-1-AP
IP result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human liver using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human liver using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 21698-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 21698-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NEDD4 antibody (21698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NEDD4 Polyclonal antibody

NEDD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21698-1-AP

Publications(61)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4, KIAA0093

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (61)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, C6 cells, HeLa cells, HT-1080 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, COS-7 cells
Positive IP detected in	C6 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 55 publications below
IHC	See 7 publications below
IF	See 13 publications below
IP	See 8 publications below
CoIP	See 3 publications below

Product Information

21698-1-AP targets NEDD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16291 Product name: Recombinant human NEDD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 970-1319 aa of BC152452 Sequence: TVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD 相同性解析による交差性が予測される生物種
Full Name	neural precursor cell expressed, developmentally down-regulated 4
Calculated molecular weight	1319 aa, 149 kDa
Observed molecular weight	115 kDa
GenBank accession number	BC152452
Gene Symbol	NEDD4
Gene ID (NCBI)	4734
RRID	AB_10858626
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P46934
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2 (PMID: 23545411). NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain (PMID: 35328067). NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

Protocols

Product Specific Protocols
IF protocol for NEDD4 antibody 21698-1-AP	Download protocol
IHC protocol for NEDD4 antibody 21698-1-AP	Download protocol
IP protocol for NEDD4 antibody 21698-1-AP	Download protocol
WB protocol for NEDD4 antibody 21698-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Adv Sci (Weinh) OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model Authors - Xiaomeng Song View Article
mouse	WB	Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice Authors - Dingfeng Zou View Article
human	WB	J Exp Clin Cancer Res PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer Authors - Liang Xu View Article
mouse	WB	J Biomed Sci Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1 Authors - Yichen Wu View Article
mouse	WB	Proc Natl Acad Sci U S A Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis. Authors - Siming Li View Article
human	WB	Theranostics PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex. Authors - Xiaoxiao Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21698-1-AP

WB analysis using 21698-1-AP

WB analysis using 21698-1-AP

WB analysis of HeLa using 21698-1-AP

WB analysis of HT-1080 using 21698-1-AP

WB analysis of A549 using 21698-1-AP

IP experiment of C6 using 21698-1-AP

IHC staining of human breast cancer using 21698-1-AP

IHC staining of human breast cancer using 21698-1-AP

IHC staining of human breast cancer using 21698-1-AP

IHC staining of human breast cancer using 21698-1-AP

IHC staining of human lung cancer using 21698-1-AP

IHC staining of human lung cancer using 21698-1-AP

IHC staining of human liver using 21698-1-AP

IHC staining of human liver using 21698-1-AP

IHC staining of human lung cancer using 21698-1-AP

IHC staining of human lung cancer using 21698-1-AP

IF Staining of U2OS using 21698-1-AP

NEDD4 Polyclonal antibody

NEDD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer

Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.

PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.