Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IP | See 1 publications below |
Product Information
13891-1-AP targets NDUFAF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4853 Product name: Recombinant human NDUFAF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-169 aa of BC033965 Sequence: MGWSQDLFRALWRSLSREVKEHVGTDQFGNKYYYIPQYKNWRGQTIREKRIVEAANKKEVDYEAGDIPTEWEAWIRRTRKTPPTMEEILKNEKHREEIKIKSQDFYEKEKLLSKETSEELLPPPVQTQIKGHASAPYFGKEEPSVAPSSTGKTFQPGSWMPRDGKSHNQ 相同性解析による交差性が予測される生物種 |
| Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2 |
| Calculated molecular weight | 169 aa, 20 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC033965 |
| Gene Symbol | NDUFAF2 |
| Gene ID (NCBI) | 91942 |
| RRID | AB_10597105 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N183 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mimitin, also named as MMTN, B17.2L, NDUFAF2 and NDUFA12L, is a small mitochondrial protein upregulated by proinflammatory cytokines at the transcriptional and protein levels. It is reported as a possible chaperone for assembly of mitochondrial complex I. The MW of Mimitin is 17-25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFAF2 antibody 13891-1-AP | Download protocol |
| IP protocol for NDUFAF2 antibody 13891-1-AP | Download protocol |
| WB protocol for NDUFAF2 antibody 13891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. | ||
J Med Genet Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome. | ||
Redox Rep Ndufaf2, a protein in mitochondrial complex I, interacts in vivo with methionine sulfoxide reductases |
