Validation Data Gallery
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Tested Applications
| Positive WB detected in | U-937 cells, Raji cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
28187-1-AP targets NCF1/p47 phox in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NCF1/p47 phox fusion protein Ag28089 相同性解析による交差性が予測される生物種 |
| Full Name | neutrophil cytosolic factor 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC002816 |
| Gene Symbol | p47phox/NCF1 |
| Gene ID (NCBI) | 653361 |
| RRID | AB_2881084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14598 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCF1/p47 phox antibody 28187-1-AP | Download protocol |
| IHC protocol for NCF1/p47 phox antibody 28187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. | ||
Oxid Med Cell Longev Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. | ||
Clin Nutr Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress. | ||
Drug Des Devel Ther Artemisinin Improves Acetylcholine-Induced Vasodilatation in Rats with Primary Hypertension | ||
Biochem Biophys Res Commun Liraglutide modulates ALCAT1-Mediated cardiolipin remodeling to improve cardiac function in obese mice | ||
Lab Invest Pathological and biological significance of the specific glycan, TRA-1-60, on aggressive gastric adenocarcinoma
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