p47 phox Polyclonal antibody

p47 phox Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28187-1-AP

Synonyms

FLJ79451, NCF 1, NCF 47K, NCF1, NCF1A, Neutrophil cytosol factor 1, neutrophil cytosolic factor 1, Nox organizer 2, Nox organizing protein 2, NOXO2, p47 phox, p47phox, p47-phox, SH3PXD1A



Tested Applications

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

28187-1-AP targets p47 phox in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p47 phox fusion protein Ag28089 相同性解析による交差性が予測される生物種
Full Name neutrophil cytosolic factor 1
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC002816
Gene symbol NCF1
Gene ID (NCBI) 653361
RRIDAB_2881084
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for p47 phox antibody 28187-1-APDownload protocol
IHC protocol for p47 phox antibody 28187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hazard Mater

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.

Authors - Feng Xiaoli
ratWB

Oxid Med Cell Longev

Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.

Authors - Mengyang Zhang
ratWB

Drug Des Devel Ther

Artemisinin Improves Acetylcholine-Induced Vasodilatation in Rats with Primary Hypertension

Authors - Xuanxuan Liu