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  • KD/KO Validated

NCAPH Polyclonal antibody

NCAPH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11515-1-AP

Synonyms

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, CND2, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11515-1-AP targets NCAPH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCAPH fusion protein Ag2076 相同性解析による交差性が予測される生物種
Full Name non-SMC condensin I complex, subunit H
Calculated molecular weight 741 aa, 83 kDa
Observed molecular weight 83-100 kDa
GenBank accession numberBC024211
Gene symbol NCAPH
Gene ID (NCBI) 23397
RRIDAB_2150003
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

Protocols

Product Specific Protocols
WB protocol for NCAPH antibody 11515-1-APDownload protocol
IHC protocol for NCAPH antibody 11515-1-APDownload protocol
IP protocol for NCAPH antibody 11515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Mol Sci

OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH.

Authors - Takuya Ogura
  • KD Validated
humanWB

Cell Death Dis

NCAPH plays important roles in human colon cancer.

Authors - Liang Yin
  • KD Validated
humanWB,IHC

Cell Cycle

NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway.

Authors - Bo Li
  • KD Validated
humanWB

J Cell Sci

Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.

Authors - Masatoshi Takagi
humanWB

Sci Rep

NCAPH is a prognostic biomarker and associated with immune infiltrates in lung adenocarcinoma.

Authors - Chao Li
humanWB,IHC

Mol Carcinog

Non-SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma.

Authors - Chengjun Sun
  • KD Validated