Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 24563-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24563-1-AP
WB result of NCAPG antibody (24563-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPG transfected HEK-293 cells.
WB analysis using 24563-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24563-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24563-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 24563-1-AP
IP result of anti-NCAPG (IP:24563-1-AP, 4ug; Detection:24563-1-AP 1:8000) with HEK-293 cells lysate 1800 ug.
IHC staining of human testis using 24563-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 24563-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 24563-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 24563-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 24563-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 24563-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NCAPG antibody (24563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24563-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CAPG, CHCG, Chromosome-associated protein G, Condensin complex subunit 3, Condensin subunit CAP G

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human testis tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 7 publications below
IHC	See 7 publications below
CoIP	See 1 publications below

Product Information

24563-1-AP targets NCAPG in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19926 Product name: Recombinant human NCAPG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 664-1015 aa of BC068467 Sequence: TLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQECFEEAFLPTLQTLANAPASSPLAEIDITNVAELLVDLTRPSGLNPQAKTSQDYQALTVHDNLAMKICNEILTSPCSPEIRVYTKALSSLELSSHLAKDLLVLLNEILEQVKDRTCLRALEKIKIQLEKGNKEFGDQAEAAQDATLTTTTFQNEDEKNKEVYMTPLRGVKATQASKSTQLKTNRGQRKVTVSARTNRRCQTAEADSESDHEVPEPESEMKMRLPRRAKTAALEKSKLNLAQFLNEDLS 相同性解析による交差性が予測される生物種
Full Name	non-SMC condensin I complex, subunit G
Calculated molecular weight	1015 aa, 114 kDa
Observed molecular weight	131 kDa
GenBank accession number	BC068467
Gene Symbol	NCAPG
Gene ID (NCBI)	64151
RRID	AB_2879610
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BPX3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for NCAPG antibody 24563-1-AP	Download protocol
IHC protocol for NCAPG antibody 24563-1-AP	Download protocol
IP protocol for NCAPG antibody 24563-1-AP	Download protocol
WB protocol for NCAPG antibody 24563-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	J Transl Med Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance Authors - Kun Zheng View Article
human	WB	Front Oncol NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma. Authors - Guangrong Zheng KD Validated View Article
mouse,human	WB,IHC,CoIP	J Transl Med NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN. Authors - Rongguiyi Zhang KD Validated View Article
human	WB	J Gastrointest Oncol NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer Authors - Hong Yang KD Validated View Article
human	WB,IHC	Cell Death Dis NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer. Authors - Lili Jiang KD Validated View Article
human	IHC	Sci Rep Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model. Authors - Yoshihiro Miyazaki View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 24563-1-AP

WB analysis of HEK-293 using 24563-1-AP

WB analysis using 24563-1-AP

WB analysis of HEK-293 using 24563-1-AP

WB analysis of HeLa using 24563-1-AP

IP experiment of HEK-293 using 24563-1-AP

IHC staining of human testis using 24563-1-AP

IHC staining of human cervical cancer using 24563-1-AP

IHC staining of human testis using 24563-1-AP

IHC staining of human cervical cancer using 24563-1-AP

IF Staining of HepG2 using 24563-1-AP

IF Staining of HepG2 using 24563-1-AP

NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma.

NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.

NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer

NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer.

Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.