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- KD/KO Validated
Myh9 Polyclonal antibody
Myh9 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, canine and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14844-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, SGC-7901 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
14844-1-AP targets Myh9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, heavy polypeptide 9, non-muscle |
| Calculated molecular weight | 226 kDa |
| Observed molecular weight | 224 kDa |
| GenBank accession number | NM_022410 |
| Gene symbol | Myh9 |
| Gene ID (NCBI) | 17886 |
| RRID | AB_2147311 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH9, also named as NMMHC-A and NMMHC-IIA, is a cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. It plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction, during cell spreading.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Myh9 antibody 14844-1-AP | Download protocol |
| IHC protocol for Myh9 antibody 14844-1-AP | Download protocol |
| IF protocol for Myh9 antibody 14844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The non-muscle-myosin-II heavy chain Myh9 mediates colitis-induced epithelium injury by restricting Lgr5+ stem cells. | ||
Theranostics Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.
| ||
Neuromolecular Med Generation and characterization of mice with Myh9 deficiency.
| ||
Clin Genet Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population. | ||
Biomed Pharmacother Tandem mass tag (TMT) proteomic analysis of fetal lungs revealed differential expression of tight junction proteins in a rat model of congenital diaphragmatic hernia. |
