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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Myelin basic protein Polyclonal antibody

Myelin basic protein Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10458

Synonyms

MBP, Myelin A1 protein, myelin basic protein

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL488-10458

    IF Staining of mouse brain using CL488-10458

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-10458

    IF Staining of mouse brain using CL488-10458

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Myelin basic protein antibody (CL488-10458) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy MBP Ready-To-Use IHC Kit. MBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10458-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10458 targets Myelin basic protein in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Myelin basic protein fusion protein Ag0713 相同性解析による交差性が予測される生物種
Full Name myelin basic protein
Calculated molecular weight 33 kDa
Observed molecular weight 14-23 kDa
GenBank accession numberBC008749
Gene symbol MBP
Gene ID (NCBI) 4155
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Myelin basic protein antibody CL488-10458Download protocol
Standard Protocols
Click here to view our Standard Protocols