Mouse Total MMP-3 ELISA Kit

Sensitivity

134.9 pg/mL

Range

250-4000 pg/mL

Reactivity

Mouse

Cat no : KE10040

Synonyms

matrix metallopeptidase 3, Mmp3, MMP-3, SLN 1, SLN1, Stmy1, STR 1, Str1



Product Information

KE10040 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total MMP3 ELISA kit is to be used to detect and quantify protein levels of endogenous Total MMP3. The assay recognizes mouse Total MMP3. An antibody specific for Total MMP3 has been pre-coated onto the microwells. The Total MMP3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Total MMP3 is added to detect the captured mouse Total MMP3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse Total MMP-3 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 134.9 pg/mL
Range 250-4000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 17392

Recovery

Sample TypeAverageRange
Mouse serum106%96%-122%
Cell culture supernatants90%73%-119%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120477.611.12.3
220967.818.31.9
3201,875.256.53.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124490.112.42.5
224992.320.72.1
3241,925.947.52.5

Background Information

MMP-3 (matrix metallopeptidase 3), also named as Stromelysin 1 or STR1, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis and tissue remodelling and in many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fbronectin, laminin, IGFBP-3, serpins, and IL-1β.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms matrix metallopeptidase 3, Mmp3, MMP-3, SLN 1, SLN1, Stmy1, STR 1, Str1

Publications

SpeciesSample TypeTitle
mouseserum

PLoS Negl Trop Dis

Dengue virus infection induces selective expansion of Vγ4 and Vγ6TCR γδ T cells in the small intestine and a cytokine storm driving vascular leakage in mice

Authors - Takeshi Kurosu