Mouse TNFRSF1B ELISA Kit0 Publications

カタログ番号: KE10061

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse TNFRSF1B ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
3.9-250 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 109% 99%-123%
Cell culture supernatants 118% 109%-128%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 108.5 3.9 3.6
2 20 33.3 0.7 2.0
3 20 15.6 0.5 3.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 92.9 8.6 9.3
2 24 30.7 2.4 7.9
3 24 14.5 1.3 9.3

製品概要
KE10061 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF1B ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF1B. The assay recognizes mouse TNFRSF1B. An antibody specific for TNFRSF1B has been pre-coated onto the microwells. The TNFRSF1B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse TNFRSF1B is added to detect the captured mouse TNFRSF1B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD120b, p75, TNF alphaR2, TNF R II, TNF R2, TNF R75, TNFalpha R2, TNFBR, Tnfr 1, Tnfr2, TNFR80, TNFRII, Tnfrsf1b
背景説明

TNFRSF1B, also known as TNFR2 or CD120b or p75, is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. Tumor necrosis factor (TNF, TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through TNFRSF1A and TNFRSF1B. TNFRSF1A is widely expressed, whereas TNFRSF1B exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells. Both TNFRSF1A and TNFRSF1B can also exist in soluble forms, probably derived by proteolytic cleavage from the cell surface forms. Various defects in the TNFRSF1B pathway, due to polymorphisms in the TNFRSF1B gene, upregulated expression of TNFRSF1B and TNFRSF1B shedding, have been implicated in the pathology of several autoimmune disorders.


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