Mouse TNFR1 ELISA Kit

Sensitivity

0.5 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10065

Synonyms

CD120a, FPF, p55, p55 R, TNF alphaR1, TNF R, TNF R I, TNF R1, TNF R55, TNFalpha R1, TNFAR, Tnfr 2, Tnfr1, TNFR60, TNFRI, TNFRp55, Tnfrsf1a



Product Information

KE10065 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MouseTNFR1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouseTNFR1. The assay recognizes mouseTNFR1. An antibody specific for mouseTNFR1 has been pre-coated onto the microwells. The mouseTNFR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouseTNFR1 is added to detect the captured mouseTNFR1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse TNFR1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernates, Tissue homogenates
Assay type Sandwich
Sensitivity 0.5 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum98%80%-123%
Cell culture supernates96%71%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120219.96.32.9
22052.21.32.5
32012.10.43.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124205.87.43.6
22451.92.14.0
32412.50.64.7

Background Information

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55-kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CD120a, FPF, p55, p55 R, TNF alphaR1, TNF R, TNF R I, TNF R1, TNF R55, TNFalpha R1, TNFAR, Tnfr 2, Tnfr1, TNFR60, TNFRI, TNFRp55, Tnfrsf1a