Mouse/Rat PDGF-AB ELISA Kit0 Publications

カタログ番号: KE10057

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse/Rat PDGF-AB ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Mouse serum, Mouse plasma, Rat serum, Rat plasma

アッセイ方法:
Sandwich

感度:
3.2 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Mouse/Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 83% 76%-97%
Rat serum 85% 76%-103%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 492.4 14.1 2.9
2 20 108.6 2.0 1.8
3 20 25.1 1.1 4.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 501.5 5.2 1.0
2 24 119.6 1.7 1.4
3 24 28.1 2.3 8.3

製品概要
KE10057 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat PDGF-AB ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat PDGF-AB. The assay recognizes mouse/rat PDGF-AB. An antibody specific for mouse/rat PDGF-AB has been pre-coated onto the microwells. The mouse/rat PDGF-AB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat PDGF-AB is added to detect the captured mouse/rat PDGF-AB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
Pdgfa
背景説明

Platelet-derived growth factor (PDGF) is one of the most abundant growth factors contained in the platelet alpha-granules and released upon platelet activation. It is also secreted by epithelial and mesenchymal cells. PDGF family consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. The PDGFR receptors are encoded by two genes: PDGFRA and PDGFRB. PDGFR is known to control growth, cell movement, angiogenesis and embryo development.


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