Mouse PCSK9 ELISA Kit0 Publications

カタログ番号: KE10050

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse PCSK9 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plamsa, Cell culture supernatants, Tissue lysates

アッセイ方法:
Sandwich

感度:
11.9 pg/mL

測定範囲:
62.5-4000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 104% 86%-125%
Cell culture supernatants 89% 80%-103%
Tissue lysates 86% 78%-93%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,867.8 84.8 4.5
2 20 440.0 14.1 3.2
3 20 99.8 5.2 5.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2,101.9 113.6 5.4
2 24 474.5 24.8 5.2
3 24 111.6 3.9 3.5

製品概要
KE10050 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PCSK9 ELISA kit is to be used to detect and quantify protein levels of endogenous PCSK9. The assay recognizes mouse PCSK9. An antibody specific for PCSK9 has been pre-coated onto the microwells. The PCSK9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PCSK9 is added to detect the captured PCSK9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
AI415265, AI747682, FH3, HCHOLA3, Narc1, Pcsk9
背景説明

PCSK9, also named as NARC1 and PC9, belongs to the peptidase S8 family. PCSK9 is expressed and secreted by several tissues but principally by the liver, small intestines, and kidney(PMID 28587771). It may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. PCSK9 is a regulator of the LDL receptor and plasma cholesterol concentrations(PMID: 26293463).


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