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CoraLite®488-conjugated Mouse IgG2b isotype control Monoclonal antibody

Mouse IgG2b isotype control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

11B8C4

Cat no : CL488-66360-3

Synonyms

Mouse IgG2b isotype control

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66360-3

    FC experiment of HeLa using CL488-66360-3

    1X10^6 HeLa cells were stained with 0.2 ug CoraLite®488 Mouse IgG2b isotype control (CL488-66360-3, Clone:11B8C4) (purple) or 0.2 ug CoraLite®488 Anti-Human BAX (CL488-60267, Clone:4G5E8) (green) or not stained (blank control) (black). Cells were fixed with 90% MeOH.

Tested Applications

Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 66360-3-Ig

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-66360-3 targets Mouse IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2b isotype control
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

0.05-0.2 ug per test is suggested for FC experiment.

Protocols

Product Specific Protocols
FC protocol for CL488 Mouse IgG2b isotype control antibody CL488-66360-3Download protocol
Standard Protocols
Click here to view our Standard Protocols