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Tested Applications
| Positive FC (Intra) detected in | |
| Positive FC detected in |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66360-3 targets IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.
| Tested Reactivity | Megathura crenulata |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | IgG2b isotype control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883318 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
0.05-0.2 ug per test is suggested for FC experiment.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IgG2b isotype control antibody CL488-66360-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |