Validation Data Gallery
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Tested Applications
| Positive WB detected in | |
| Positive IHC detected in | Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| ChIP | See 2 publications below |
Product Information
66360-3-Ig targets Mouse IgG2b isotype control in WB, IHC, FC, chIP, ELISA applications and shows reactivity with megathura crenulata samples.
| Tested Reactivity | megathura crenulata |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | Mouse IgG2b isotype control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| IHC protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma. | ||
Cell Death Dis E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway. | ||
Aging Dis Age-related Loss of miR-124 Causes Cognitive Deficits via Derepressing RyR3 Expression | ||
Immunity Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity | ||
Nat Commun Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire | ||
J Virol The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating |