Mouse IgG2b isotype control Monoclonal antibody

Mouse IgG2b isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2b


Megathura crenulata







Cat no : 66360-3-Ig


IgG2b isotype, KLH, mouse IgG control, Mouse IgG2b control, Mouse IgG2b isotype control

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  • WB analysis using 66360-3-Ig

    WB analysis using 66360-3-Ig

    Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 66360-3-Ig

    FC experiment of Jurkat using 66360-3-Ig

    1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected in
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

ChIPSee 2 publications below

Product Information

66360-3-Ig targets Mouse IgG2b isotype control in WB, FC, chIP, ELISA applications and shows reactivity with Megathura crenulata samples.

Tested Reactivity Megathura crenulata
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name Mouse IgG2b isotype control
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).


Product Specific Protocols
WB protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
FC protocol for Mouse IgG2b isotype control antibody 66360-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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