Mouse IgG2b isotype control Monoclonal antibody
Mouse IgG2b isotype control Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Megathura crenulata
Applications
WB, FC, chIP, ELISA
Conjugate
Unconjugated
CloneNo.
11B8C4
Cat no : 66360-3-Ig
Synonyms
"Mouse IgG2b isotype control Antibodies" Comparison
View side-by-side comparison of Mouse IgG2b isotype control antibodies from other vendors to find the one that best suits your research needs.
Applications:
Tested Applications
| Positive WB detected in | |
| Positive FC detected in | Jurkat cells |
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| ChIP | See 2 publications below |
Product Information
66360-3-Ig targets Mouse IgG2b isotype control in WB, FC, chIP, ELISA applications and shows reactivity with Megathura crenulata samples.
| Tested Reactivity | Megathura crenulata |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein |
| Full Name | Mouse IgG2b isotype control |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| FC protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway. | ||
Front Oncol The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma. |
