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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated Mouse-IgG Polyclonal antibody

Mouse-IgG Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-10283

Synonyms

Validation Data Gallery

Applications:
  • IHC staining of human colon cancer using Biotin-10283

    IHC staining of human colon cancer using Biotin-10283

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66031-1-Ig (alpha tubulin antibody) and Biotin-10283 (Biotin-Rabbit anti-Mouse IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

Biotin-10283 targets Mouse-IgG in IHC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name Mouse-IgG
Calculated molecular weight 155 kDa
Observed molecular weight 25 kDa, 50-55 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_2918932
Conjugate Biotin
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detectes all the heavy and light chains of total mouse IgG.

Protocols

Product Specific Protocols
IHC protocol for Biotin Mouse-IgG antibody Biotin-10283Download protocol
Standard Protocols
Click here to view our Standard Protocols