Mouse IL-2 ELISA Kit

Sensitivity

0.5 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse

Cat no : KE10004

Synonyms

Il 2, Il2, IL-2, interleukin 2, interleukin-2



Product Information

KE10004 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-2. The assay recognizes mouse IL-2. An antibody specific for IL-2 has been pre-coated onto the microwells. The IL-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-2 is added to detect the captured IL-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.5 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16183

Recovery

Sample TypeAverageRange
Mouse serum111%99%-120%
Cell culture supernatants107%94%-113%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1201,176.774.66.3
220358.517.44.8
32064.13.55.4

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,090.888.58.1
224331.528.78.7
32458.32.94.9

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signal is mediated through heterodimerization of the IL2Rβ and Il-2Rγ receptor subunits.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il 2, Il2, IL-2, interleukin 2, interleukin-2

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

ACS Nano

Self-Assembly of Podophyllotoxin-Loaded Lipid Bilayer Nanoparticles for Highly Effective Chemotherapy and Immunotherapy via Downregulation of Programmed Cell Death Ligand 1 Production.

Authors - Rui Wang
mousecell culture supernatants

J Immunother Cancer

ANKRD22 is a potential novel target for reversing the immunosuppressive effects of PMN-MDSCs in ovarian cancer

Authors - Huanhuan Chen
mousecell culture supernatants

Mol Pharm

Cationic Nanoparticulate System for Codelivery of MicroRNA-424 and Podophyllotoxin as a Multimodal Anticancer Therapy.

Authors - Rui Wang
mousecell culture supernatants

Inflammation

Silencing TLR4/MyD88/NF-κB Signaling Pathway Alleviated Inflammation of Corneal Epithelial Cells Infected by ISE.

Authors - Liucheng Wu
mouseplasma

Dermatol Ther

Ameliorative effect of green odor against UVB-induced immunosuppression of contact hypersensitivity.

Authors - Yurika Yamate
mouseserum

Biomed Rep

Effect of Coriolus versicolor glucan on the stimulation of cytokine production in sarcoma-180-bearing mice.

Authors - Annoor Awadasseid