Mouse IL-33 ELISA Kit
Cat no : KE10054
Synonyms
9230117N10Rik, Il 33, Il1f11, Il33, IL-33, interleukin 33, NF HEV
Validation Data Gallery
Product Information
KE10054 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL33 ELISA kit is to be used to detect and quantify protein levels of endogenous IL33. The assay recognizes mouse IL33. An antibody specific for IL33 has been pre-coated onto the microwells. The IL33 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL33 is added to detect the captured mouse IL33 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-33 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.7 pg/mL |
| Range | 62.5-4000 pg/mL, 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 77125 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 88% | 71%-104% |
| Cell culture supernatants | 82% | 74%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 59.5 | 2.8 | 4.7 |
| 2 | 20 | 228.4 | 12.8 | 5.6 |
| 3 | 20 | 940.2 | 54.1 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 62.4 | 5.5 | 8.9 |
| 2 | 24 | 220.8 | 22.0 | 10.0 |
| 3 | 24 | 892.1 | 75.0 | 8.4 |
Background Information
Interleukin-33 (IL-33) is a tissue-derived nuclear cytokine from the IL-1 family abundantly expressed in endothelial cells, epithelial cells and fibroblast-like cells, both during homeostasis and inflammation. IL-33 activates many immune cell types involved in type-2 immunity and allergic inflammation, including ILC2s, mast cells, Th2 cells, eosinophils, basophils, dendritic cells and alternatively activated macrophages (AAM). As a cytokine, IL-33 interacts with the receptors ST2 (also known as IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. IL-33 is also effective in reversing Alzheimer-like symptoms in APP/PS1 mice, by reversing the buildup and preventing the new formation of amyloid plaques.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 9230117N10Rik, Il 33, Il1f11, Il33, IL-33, interleukin 33, NF HEV |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Inflamm Res Sodium Butyrate Ameliorates Type 2 Diabetes-Related Sarcopenia Through IL-33-Independent ILC2s/IL-13/STAT3 Signaling Pathway | ||
Cell Death Dis Macrophages originated IL-33/ST2 inhibits ferroptosis in endometriosis via the ATF3/SLC7A11 axis | ||
Immunol Lett Role of G-protein-coupled estrogen receptor in the pathogenesis of chronic asthma |