Mouse IL-1 beta ELISA Kit

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10003

Synonyms

Il 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta



Product Information

KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes mouse IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.NB: precursor and active form can be detected by this kit.

Product name Mouse IL-1 beta ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, tissue lysates
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum102%91%-123%
Cell culture supernatants113%89%-126%
Cell lysates104%82%-128%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120520.017.03.3
220159.55.63.5
32029.10.51.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124485.520.14.1
224156.64.83.1
32428.51.34.6

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta

Publications

SpeciesSample TypeTitle
mousetissue homogenates

J Infect Dis

TIPE2 suppresses Pseudomonas aeruginosa keratitis by inhibiting NF-κB signaling and the infiltration of inflammatory cells.

Authors - Qun Wang
mousecell culture supernatants

Cells

Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

Authors - Takayuki Inoue
mouseplasma

Biomed Pharmacother

Chaihu-Shugan-San and absorbed meranzin hydrate induce anti-atherosclerosis and behavioral improvements in high-fat diet ApoE-/- mice via anti-inflammatory and BDNF-TrkB pathway.

Authors - Lan Li
mouseserum

Biomed Pharmacother

Shikonin attenuates acetaminophen-induced acute liver injury via inhibition of oxidative stress and inflammation.

Authors - Hongli Guo
mouseserum

Oxid Med Cell Longev

Catalpol-Induced AMPK Activation Alleviates Cisplatin-Induced Nephrotoxicity through the Mitochondrial-Dependent Pathway without Compromising Its Anticancer Properties.

Authors - Jiangnan Zhang
mousecell culture supernatants

J Immunol Res

Ganoderma lucidum Spore Polysaccharide Inhibits the Growth of Hepatocellular Carcinoma Cells by Altering Macrophage Polarity and Induction of Apoptosis.

Authors - Ming Song