製品名:

Mouse IL1-beta ELISA Kit

試験方法:

1 X 96 well plate

測定サンプル対象:

Serum, Plasma, Cell culture supernatants, Cell lysates

アッセイ方法:

Sandwich

感度:

1.0 pg/mL

測定範囲:

7.8-500 pg/mL

測定可能動物種:

Mouse

適用アプリケーション:

Sandwich ELISA

回収率:

Sample Type	Average	Range
Mouse serum	102%	91%-123%
Cell culture supernatants	113%	89%-126%
Cell lysates	104%	82%-128%

製品概要

KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1β ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1β. The assay recognizes mouse IL-1β. A monoclonal antibody specific for IL-1β has been pre-coated onto the microwells. The IL-1β protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL-1β is added to detect the captured IL-1β protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

保存方法:

All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:

Il 1b, IL 1beta, IL-1, IL1 BETA, Il1b, IL-1b, IL1beta, interleukin 1 beta