Mouse/Rat IGF1 ELISA Kit2 Publications

カタログ番号: KE10032

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse/Rat IGF1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell lysates, Tissue lysates

アッセイ方法:
Sandwich

感度:
1.3 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Mouse/Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 105% 95%-119%
Rat serum 103% 79%-117%
Tissue lysates 103% 87%-121%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 550.2 16.0 2.9
2 20 164.8 6.7 4.1
3 20 78.0 5.1 6.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 501.3 38.4 7.7
2 24 138.5 14.1 10.2
3 24 56.0 6.1 10.8

製品概要
KE10032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse/Rat IGF1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat IGF1. The assay recognizes mouse/rat IGF1. An antibody specific for mouse/rat IGF1 has been pre-coated onto the microwells. The mouse/rat IGF1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat IGF1 is added to detect the captured mouse/rat IGF1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
C730016P09Rik, Igf 1, Igf I, Igf1, insulin like growth factor 1
背景説明

Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.


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