Mouse Adiponectin ELISA Kit0 Publications

カタログ番号: KE10044

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse Adiponectin ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell Culture Supernates, Tissue homogenates,

アッセイ方法:
Sandwich

感度:
6.9 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 102% 91%-122%
Mouse fat tissue Homogenates 96% 82%-106%
Cell Culture Supernates 93% 77%-104%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 940.5 43.0 4.6
2 20 224.3 8.3 3.7
3 20 55.5 2.9 5.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,009.9 79.9 7.9
2 24 237.4 10.2 4.3
3 24 56.5 3.1 5.5

製品概要
KE10044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes mouse Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
30kDa, Acdc, Acrp30, adipo, Adipoq, apM1, APN, GBP28
背景説明

Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.


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