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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Midkine Polyclonal antibody

Midkine Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28546-1-AP

Synonyms

ARAP, FLJ27379, MDK, Midkine, MK, MK1, NEGF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Midkine Polyclonal antibody (28546-1-AP)

    WB analysis using 28546-1-AP

    Mouse embryo tissues were subjected to SDS PAGE followed by western blot with 28546-1-AP (Midkine antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Midkine Polyclonal antibody (28546-1-AP)

    IHC staining of human pancreas cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Midkine Polyclonal antibody (28546-1-AP)

    IHC staining of human pancreas cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Midkine Polyclonal antibody (28546-1-AP)

    IHC staining of human lung cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Midkine Polyclonal antibody (28546-1-AP)

    IHC staining of human lung cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Midkine Polyclonal antibody (28546-1-AP)

    IHC staining of human stomach cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Midkine Polyclonal antibody (28546-1-AP)

    IHC staining of human stomach cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse embryo tissue
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28546-1-AP targets Midkine in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Midkine fusion protein Ag29185 相同性解析による交差性が予測される生物種
Full Name midkine (neurite growth-promoting factor 2)
Calculated molecular weight 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC011704
Gene symbol Midkine
Gene ID (NCBI) 4192
RRIDAB_2881168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Midkine antibody 28546-1-APDownload protocol
IHC protocol for Midkine antibody 28546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EBioMedicine

Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis

Authors - Masahiro Hashimoto
View Article