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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

Mecr Polyclonal antibody

Mecr Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51025-2-Ig

Synonyms

AI195831, Mecr, Nrbf1

Validation Data Gallery

Applications:
  • WB analysis of mouse skeletal muscle using 51025-2-Ig

    WB analysis of mouse skeletal muscle using 51025-2-Ig

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (Mecr antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 51025-2-Ig

    WB analysis of mouse heart using 51025-2-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 51025-2-Ig (Mecr antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 51025-2-Ig

    IHC staining of mouse skeletal muscle using 51025-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (Mecr antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 51025-2-Ig

    IHC staining of mouse skeletal muscle using 51025-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 51025-2-Ig (Mecr antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

51025-2-Ig targets Mecr in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitochondrial trans-2-enoyl-CoA reductase
Calculated molecular weight 40 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC003864
Gene symbol Mecr
Gene ID (NCBI) 26922
RRIDAB_1167469
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Mecr antibody 51025-2-IgDownload protocol
IHC protocol for Mecr antibody 51025-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Non-electron transfer chain mitochondrial defects differently regulate HIF-1α degradation and transcription.

Authors - Antonina N Shvetsova
  • KD Validated
View Article
mouseWB

Biochem Biophys Res Commun

The mitochondrial fatty acid synthesis (mtFASII) pathway is capable of mediating nuclear-mitochondrial cross talk through the PPAR system of transcriptional activation.

Authors - Parl Angelika A
View Article