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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    WB analysis of mouse skeletal muscle using 16052-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16052-1-AP (MYLPF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    WB analysis of mouse skeletal muscle using 16052-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16052-1-AP (MYLPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using MYLPF Polyclonal antibody (16052-1-AP)

    IHC staining of human skeletal muscle using 16052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16052-1-AP (MYLPF Antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MYLPF Polyclonal antibody

MYLPF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16052-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HUMMLC2B, MLC2B, MRLC2, MYL11, MYLPF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16052-1-AP targets MYLPF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLPF fusion protein Ag9013 相同性解析による交差性が予測される生物種
Full Name myosin light chain, phosphorylatable, fast skeletal muscle
Calculated molecular weight 169 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC012571
Gene Symbol MYLPF
Gene ID (NCBI) 29895
RRIDAB_2148036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYLPF antibody 16052-1-APDownload protocol
IHC protocol for MYLPF antibody 16052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Gene Transfer of Skeletal Muscle-Type Myosin Light Chain Kinase via Adeno-Associated Virus 6 Improves Muscle Functions in an Amyotrophic Lateral Sclerosis Mouse Model.

Authors - Ryohei Oya
View Article
mouseWB

Mol Cell Biol

MUNC, an Enhancer RNA Upstream from the MYOD Gene, Induces a Subgroup of Myogenic Transcripts in trans Independently of MyoD.

Authors - Magdalena A Cichewicz
View Article