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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYL7 Recombinant antibody

MYL7 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C5

Cat no : 81570-1-RR

Synonyms

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

Validation Data Gallery

Applications:
  • WB analysis using 81570-1-RR

    WB analysis using 81570-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81570-1-RR (MYL7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 81570-1-RR

    IHC staining of mouse heart using 81570-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81570-1-RR (MYL7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYL7 antibody included

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL7 Ready-To-Use IHC Kit. MYL7 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

81570-1-RR targets MYL7 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MYL7 fusion protein Ag11058 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 7, regulatory
Calculated molecular weight 175 aa, 19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC027915
Gene symbol MYL7
Gene ID (NCBI) 58498
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

Protocols

Product Specific Protocols
WB protocol for MYL7 antibody 81570-1-RRDownload protocol
IHC protocol for MYL7 antibody 81570-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols