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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYL3 Monoclonal antibody

MYL3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4B2

Cat no : 66286-1-Ig

Synonyms

Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1

Validation Data Gallery

Applications:
  • WB analysis using 66286-1-Ig

    WB analysis using 66286-1-Ig

    Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 66286-1-Ig

    WB analysis of human skeletal muscle using 66286-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 66286-1-Ig

    WB analysis of mouse heart using 66286-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 66286-1-Ig

    IHC staining of human heart using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

    Ready-to-use IHC kits available with MYL3 antibody included
  • IHC staining of human heart using 66286-1-Ig

    IHC staining of human heart using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

    Ready-to-use IHC kits available with MYL3 antibody included
  • IHC staining of human skeletal muscle using 66286-1-Ig

    IHC staining of human skeletal muscle using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

    Ready-to-use IHC kits available with MYL3 antibody included
  • IHC staining of human skeletal muscle using 66286-1-Ig

    IHC staining of human skeletal muscle using 66286-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

    Ready-to-use IHC kits available with MYL3 antibody included

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse heart tissue, human heart tissue, pig heart tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYL3 Ready-To-Use IHC Kit. MYL3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66286-1-Ig targets MYL3 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYL3 fusion protein Ag24535 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated molecular weight 22 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC009790
Gene symbol MYL3
Gene ID (NCBI) 4634
RRIDAB_2881669
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Protocols

Product Specific Protocols
WB protocol for MYL3 antibody 66286-1-IgDownload protocol
IHC protocol for MYL3 antibody 66286-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols