Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissues |
| Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
10324-1-AP targets MYL12B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MYL12B fusion protein Ag0398 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, light chain 12B, regulatory |
| Calculated molecular weight | 20 kDa |
| Observed molecular weight | 18-20 kDa |
| GenBank accession number | BC004994 |
| Gene Symbol | MYL12B |
| Gene ID (NCBI) | 103910 |
| RRID | AB_2166830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14950 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially "activates" the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.(PMID: 22136066)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYL12B antibody 10324-1-AP | Download protocol |
| IHC protocol for MYL12B antibody 10324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. | ||
Biochemistry (Mosc) Western Blotting-Based Quantitative Measurement of Myosin II Regulatory Light Chain Phosphorylation in Small Amounts of Non-muscle Cells. | ||
BMC Cell Biol Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. | ||
J Pept Sci Design of peptidase-resistant peptide inhibitors of myosin light chain kinase. | ||
Biochemistry (Mosc) Long-Term Experimental Hyperglycemia Does Not Impair Macrovascular Endothelial Barrier Integrity and Function in vitro | ||
BMC Microbiol Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice |