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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYH4-Specific Polyclonal antibody

MYH4-Specific Polyclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 20140-1-AP

Synonyms

MYH2B, MYH4, MYH4-Specific, MyHC 2B, MyHC IIb, Myosin 4, Myosin heavy chain 2b, Myosin heavy chain 4, Myosin heavy chain IIb

Validation Data Gallery

Applications:
  • WB analysis of human skeletal muscle using 20140-1-AP

    WB analysis of human skeletal muscle using 20140-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 20140-1-AP

    WB analysis using 20140-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 20140-1-AP

    IHC staining of human skeletal muscle using 20140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 20140-1-AP

    IHC staining of human skeletal muscle using 20140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse skeletal muscle using 20140-1-AP

    IHC staining of mouse skeletal muscle using 20140-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 20140-1-AP

    IF Staining of mouse skeletal muscle using 20140-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH4-Specific antibody (20140-1-AP) at dilution of 1:200, MYH2-specific antibody (55069-1-AP, green), MYH7-specific antibody (22280-1-AP, blue).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20140-1-AP targets MYH4-Specific in WB,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 4, skeletal muscle
Calculated molecular weight 223 kDa
Observed molecular weight 230 kDa
GenBank accession numberNM_017533
Gene symbol MYH4
Gene ID (NCBI) 4622
RRIDAB_10666434
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.

Protocols

Product Specific Protocols
WB protocol for MYH4-Specific antibody 20140-1-APDownload protocol
IHC protocol for MYH4-Specific antibody 20140-1-APDownload protocol
IF protocol for MYH4-Specific antibody 20140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.

Authors - Andres Ramirez-Martinez
View Article
mouseIF

Development

The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition

Authors - Tiffany L. Dill
View Article
mouseIF

Am J Pathol

Klotho null mutation involvement in adenosine A2B receptor-related skeletal muscle degeneration

Authors - Hung-Liang Pai
View Article
mouseIF

Development

The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.

Authors - Tiffany L Dill
View Article
mouseWB

Int J Mol Sci

Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle

Authors - Xueqiao Wang
View Article
mouseWB

Sci Rep

Estrogen/ERR-α signaling axis is associated with fiber-type conversion of upper airway muscles in patients with obstructive sleep apnea hypopnea syndrome.

Authors - H H Chen
View Article