MYH14 Polyclonal antibody
MYH14 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 20716-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COLO 320 cells, human skeletal muscle tissue, mouse colon tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue |
Positive IP detected in | mouse kidney tissue |
Positive IF detected in | Hela cells |
Positive FC detected in | HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 5 publications below |
IHC | See 2 publications below |
IF | See 8 publications below |
Product Information
20716-1-AP targets MYH14 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | myosin, heavy chain 14 |
Calculated molecular weight | 228 kDa |
Observed molecular weight | 228 kDa |
GenBank accession number | NM_024729 |
Gene symbol | MYH14 |
Gene ID (NCBI) | 79784 |
RRID | AB_10859247 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.
Protocols
Product Specific Protocols | |
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WB protocol for MYH14 antibody 20716-1-AP | Download protocol |
IF protocol for MYH14 antibody 20716-1-AP | Download protocol |
IP protocol for MYH14 antibody 20716-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions. | ||
J Cell Biol Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane | ||
Clin Genet Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population. | ||
Stem Cells Int Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway. | ||
Mol Biol Cell Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli. | ||
Life (Basel) Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy. |