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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTSS1L Polyclonal antibody

MTSS1L Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27832-1-AP

Synonyms

ABBA 1, metastasis suppressor 1 like, MTSS1 like protein, MTSS1L

Validation Data Gallery

Applications:
  • WB analysis using 27832-1-AP

    WB analysis using 27832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27832-1-AP (MTSS1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 27832-1-AP

    IP experiment of Jurkat using 27832-1-AP

    IP result of anti-MTSS1L (IP:27832-1-AP, 4ug; Detection:27832-1-AP 1:300) with Jurkat cells lysate 4000 ug.

  • IHC staining of mouse brain using 27832-1-AP

    IHC staining of mouse brain using 27832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27832-1-AP

    IHC staining of mouse brain using 27832-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27832-1-AP (MTSS1L antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 27832-1-AP

    IF Staining of MCF-7 using 27832-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MTSS1L antibody (27832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 27832-1-AP

    IF Staining of MCF-7 using 27832-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27832-1-AP (MTSS1L antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

27832-1-AP targets MTSS1L in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTSS1L fusion protein Ag27216 相同性解析による交差性が予測される生物種
Full Name metastasis suppressor 1-like
Observed molecular weight 68-70 kDa
GenBank accession numberBC002770
Gene symbol MTSS1L
Gene ID (NCBI) 92154
RRIDAB_2880986
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MTSS1L antibody 27832-1-APDownload protocol
IHC protocol for MTSS1L antibody 27832-1-APDownload protocol
IF protocol for MTSS1L antibody 27832-1-APDownload protocol
IP protocol for MTSS1L antibody 27832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Discov

m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

Authors - Wei Zhuo
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

MicroRNA-23a promotes colorectal cancer cell migration and proliferation by targeting at MARK1.

Authors - Xiaoli Tang
View Article