Validation Data Gallery

Filter: AllWB

WB analysis of HEK-293 using 16694-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16694-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16694-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16694-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16694-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16694-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16694-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MTRF1L Polyclonal antibody

MTRF1L Polyclonal Antibody for WB, ELISA

Cat No. 16694-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

MRF1L, mtRF1a, MTRF1L

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

HEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

16694-1-AP targets MTRF1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MTRF1L fusion protein Ag10081 相同性解析による交差性が予測される生物種
Full Name	mitochondrial translational release factor 1-like
Calculated molecular weight	380 aa, 44 kDa
Observed molecular weight	40-46 kDa
GenBank accession number	BC011873
Gene Symbol	MTRF1L
Gene ID (NCBI)	54516
RRID	AB_2878301
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UGC7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTRF1L, also named as Peptide chain release factor 1-like, mitochondrial, is a 380 amino acid protein, which belongs to the prokaryotic/mitochondrial release factor family and locates in the mitochondrial. MTRF1L directs the termination of translation in response to the peptide chain termination codons UAA and UAG. MTRF1L is expressed in skeletal muscle.

Protocols

Product Specific Protocols
WB protocol for MTRF1L antibody 16694-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice. Authors - Takumi Yokokawa View Article
mouse	WB	Physiol Rep Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. Authors - Takumi Yokokawa View Article
rat	WB	Sci Total Environ Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects Authors - Dongjie Li View Article
rat	WB	J Hazard Mater ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis Authors - Zhiyuan Tian View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 16694-1-AP

WB analysis of HeLa using 16694-1-AP

WB analysis of HEK-293 using 16694-1-AP

WB analysis of HepG2 using 16694-1-AP

WB analysis of HeLa using 16694-1-AP

WB analysis of HEK-293 using 16694-1-AP

WB analysis of HEK-293 using 16694-1-AP

MTRF1L Polyclonal antibody

MTRF1L Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis