Validation Data Gallery
|Positive WB detected in
|MCF-7 cells, MDA-MB-453s cells, HeLa cells
|Positive IHC detected in
|mouse testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Sample-dependent, check data in validation data gallery
20657-1-AP targets mTOR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, chicken, rat, mouse, fish, hamster, pig, canine, bovine
|Host / Isotype
|Rabbit / IgG
|FK506 binding protein 12-rapamycin associated protein 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359). The antibody is specific to MTOR.
Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.
J Exp Med
Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.
Cell Death Differ
PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma.
FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma.
Autophagy and post-ischemic conditioning in retinal ischemia.
Int J Biol Macromol
Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient mice